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DECREE 29 December 2010 MiPAAFNational provisions for the implementation of Regulation (EC) no. 1744/2006 of the Commission of 24th November 2006, which establishes the modalities concerning the aid for silkworms

Article 1
1. This decree regulates the aid provided for the breeding of silkworms as per regulations (CE) n.1234 / 2007 and (CEE) n. 922/1972 of the Council, as well as the application rules set out in Regulation (EC) no. 1744/2006 of the Commission.

Article 2
1. For the purposes of this decree, the following definitions apply:
a) "Commission Regulation" means Commission Regulation (EC) No. 1744/2006 of 24 November 2006;
b) "Single CMO Regulation", Regulation (EC) no. 1234/2007 of the Council of 22 October 2007 on the common organization of agricultural markets and specific provisions for certain agricultural products;
(c) "AGEA Coordinamento", the Coordination Body - AGE Deputy for the management of the aid scheme for silkworm farmers;
d) «Paying agency», - Paying agency - AGEA, as well as regional paying agencies recognized in accordance with current regulations;
e) «Recognized bodies», the bodies, public or private, recognized, pursuant to art. 5 of the Commission Regulation, for the purpose of production and delivery of silk dibachi seed frames, as well as for the withdrawal of cocoons obtained from silkworm breeding.

Article 3
Marketing campaign
1. The marketing campaign begins on April 1st and ends on March 31st of the following year, as established in art. 3, letterab), point ii), of the single CMO regulation.

Article 4
Granting of help
1. Help is granted to silkworm breeders under the conditions set out in art. 2 of Regulation (EEC) no. 922/1972 of the Council and art. 2 of the Commission regulation.
2. The amount of the aid is fixed in art. 111, paragraph 3. of the single CMO regulation.

Article 5
Aid application
1. Help is granted to silkworm breeding farms who submit an application to the paying agency within the deadlines set by art. 3, paragraph 1. of the Commission regulation.
2. AGEA Coordinamento fixes the modalities for the presentation of the aid request based on the provisions of art. 4 of the Commission regulation.

Article 6
Recognized bodies
1. The Bodies referred to in art. 6, first and second indents of the Commission regulation and art. 2, paragraph 2. of Regulation (EEC) no. 922/72, are recognized by the regions.
2. The recognition referred to in paragraph 1. concerns:
a) Bodies authorized to deliver the frames;
b) Bodies authorized to collect mature cocoons.
The Bodies referred to in letter a) must meet the conditions and minimum requirements listed below:
1) use of duly certified eggs free of inherited diseases and endowed with vitality;
2) use of incubation chambers suitable for breeding;
3) have suitable equipment to maintain adequate hygienic-sanitary conditions.
The Bodies referred to in letter b) must meet the conditions and minimum requirements listed below:
1) use of suitable instruments for weighing sorted cocoons with characteristics as per art. 2, letter b)
of the Commission regulation;
2) use of rooms suitable for cocoons;
3) have facilities suitable for the treatment of conservation cocoons or, alternatively, undertake to market the cocoons within 10 days of sizing or to be used for reproduction by transferring them to the seed-silkworm production center of the beekeeping and silkworm farming unit at the Council for research and experimentation in agriculture (CRA) or to another body recognized by the same CRA
3. The Bodies referred to in paragraphs 1 and 2 must keep accounts, in accordance with art. 5, paragraph 1. first and second tract of the Commission regulation and must issue the certificates provided for in art. 6, first and second tract of the Commission regulation.
4. To maintain recognition, the bodies referred to in paragraphs 1 and 2 must submit to the regions, before the start of each marketing year, a declaration certifying the fulfillment of the recognition requirements, as well as the commitment to submit to the checks provided for by the EU and national regulations governing the sector. in question.

Article 7
Payment of help
1. The paying agency will provide the aid provided for by the Commission regulation.

Article 8.
1. AGEA Coordinazione ensures the implementation of a control program in compliance with EU and national provisions, in order to guarantee the regular payment of the aid in question.

Article 9.
1. AGEA Coordinazione provides the communications required by regulation (CEE) n. 1496/77 of the Commission of 4 July 1977.

Article 10.
Final provisions
1. This ministerial decree applies from the 2011/2012 marketing year.
2. The ministerial decree of 21 June 1982 is repealed.

Rome, December 29, 2010

Technical-scientific support for the application of the decree of 29 December 2010

CRA-API - Apiculture and Bachiculture Research Unit

Delivery of the frames
The silkworm eggs, both produced in Italy (by CRA-API, which is the only entity capable of successfully completing the production of silkworm eggs, being in possession of the parent lines and the know-how and equipment for production), and imported from abroad, must be accompanied by specific documentation.
The documentation concerns:
1) the name of the manufacturer and the country of origin.
2) the production season (in fact, the eggs cannot be kept for more than a year, therefore they must be laid in the spring or summer-autumn of the year preceding that of delivery to the farmers).
3) the name of the breed or polyhybrid strain that is bred.
4) the weight and number of eggs contained in the frame (the eggs can be subject to weight during storage for the process of breathing and gas exchange with the outside, therefore the weight is defined by the manufacturer at the time of packaging).
5) the health status of the eggs, i.e. the declaration of the producer that the eggs are not carriers of the hereditary disease, whose etiological agent (Nosema bombycis) is transmitted from the mother butterfly to the progeny (the absence of the disease is ascertained by microscopic examination on the mother butterflies) .
6) the expected hatching percentage (i.e. the number of larvae that are presumed to be born from the eggs at the end of the incubation). This percentage should be between trail 90 and 95%, and is also strongly influenced by the incubation conditions (for example, it can drop considerably if the environmental humidity is not adequate or the incubation temperature is too high). The hatching is calculated at the end of three days (on the first day the spies appear, that is the first larvae, in the second day there is the actual birth, on the third day there is the birth of a percentage of late lators). quantity of eggs born proceed in this way:
at least 4-5 groups of 100 eggs are taken at several points on each frame. The gray eggs are the unborn ones, the yellow-white ones are the shells of the born ones. If there are 2 gray eggs out of 100, the birth is 98%, if there are 5 out of 100 it is 95% and so on.

Incubation chamber
The incubation chamber must be an environment with the following characteristics:
1) must be separated from the other cocoon breeding and / or storage spaces.
2) the dimensions may vary according to the number of frames placed in the incubation (at the limit it could also be a thermostatic cabinet).
3) must guarantee the maintenance of the following environmental conditions: temperature 25 ° C + 2 ° C, humidity 75-80%, artificial or natural lighting, with alternating day-night photoperiod and with the possibility of obscuring any sources of natural lighting (windows), in a to get dark in the day before the birth (bleaching phase of the eggs).
4) the frames must be arranged for hatching on flat surfaces (tables, shelves, frames) that guarantee the passage of air between one frame and the other (i.e. they can be stacked on overlapping shelves, but with a minimum space of 5 cm by air circulation).
5) The floor of the incubation chamber must be washable with disinfectant solutions based on quaternary ammonium salts, diluted solutions of sodium hypochlorite or other detergents with equivalent disinfectant power.

The equipment in the incubation chamber must be washable with quaternary ammonium salts, diluted solutions of sodium hypochlorite or other detergents with equivalent disinfectant power before use (in the case of non-corrodible plastic equipment). If you have frames or wooden tools, these must be subjected to the action of ultraviolet rays (exposure to the open air), for the two or three days before the start of the incubation.

Withdrawal of cocoons
Instrumentation for weighing cocoons
1) for cocoon weighing, the body destined for storage must be equipped with a scale capable of weighing quantities of cocoon between 0 and 50 kg (assuming that the average production per frame is 20 kg minimum and can reach 40 kg as maximum ).

Rooms suitable for conservation
1) the rooms suitable for the cocoon conservation are premises for non-mixed use with other farmed rooms, where a low humidity (70%) can be maintained and with windows protected by nets to prevent the passage of rodents and other insects, however provided closing time. Good ventilation must be ensured (also simply by opening the windows) and the cocoons must be turned over during storage or stored in hanging net bags.

Suitable structures for the treatment of cocoons for conservation
The treatment of the cocoon for conservation purposes can be:
1) heat treatment or drying in the dryer in order to kill the chrysalis and preserve the cocoon for traditional reeling.
2) cutting the cocoon (manual or mechanical) to obtain the fresh bark to be carded, separating it manually or mechanically from the chrysalis, which in turn can be dried, used as fertilizer or as peranimal feed. The fresh bark can be stored, until commercialization, in the same conditions indicated for the dried cocoon.
Therefore the structures suitable for cocoon treatment are:
a) room with dryer; b) room where the cocoon is cut with windows, boards and adequate lighting in the case of manual cutting or cocoon-cutting machine in the case of mechanical cutting.
The bodies authorized to deliver the seed-silkworm frames and to the withdrawal of the cocoon are obliged to keep the accounts and to issue delivery / collection certificates, according to the provisions of art. 5 and 6 of the EC Reg. 1744/2006.
General note on breeding methods with modern and economically sustainable technologies. Technical aspects on mixing the seed-seed coming from different frames
Two distinct births can occur from a canvas. As explained for the purpose of incubation, the hatching of the eggs is not perfectly contemporary.
Therefore, breeders who produce more than one frame feed a first part of larvae in the hatching day of the eggs and the day after a second birth. This allows you to feed the newly hatched larvae, without making them wait a day on an empty stomach and optimizes the percentage of survival. Therefore at least two fractions of larvae are obtained from each frame, which, starting feeding on two different days, cannot be recompacted in a single breeding area.
Furthermore, since the surface covered by the larvae in the first age is very limited (it reaches 0.8 sq m at the end of the first age for each frame) it is good that the larvae also belonging to different frames are raised together. This allows to limit the desiccation of the leaf, which is distributed in small pieces during the first larval ages. A single layer desiccates much less than many small piles, allowing the larvae to have a much more continuous feeding (when the leaf dries up, the small larvae cease to eat).
The transfer of the larvae to the piece is carried out by bringing the larvae from the III to IV age mulberry surami distributed on the larvae themselves. We proceed by gradually enlarging the piece, moving the larvae with the branches and creating continuous rows in a shed or in a tunnel, by placing them on special racks for mechanized breeding. In all types of breeding, the economy of the breeding itself is determined by the speed with which feeding and cleaning operations are completed. In the piece, branch stacks are formed, and, starting from the lowest layer, the larvae independently go back to the higher layers, after consuming the leaf of the lower levels. It is clear, that when the operator passes with the load of the branches, to distribute the meals, he must lay them on the rows of branches (piece) without continuity solution, both because the branches have variable dimensions, and must not be
cut into small pieces, both because the larvae must be able to move independently within the leaf mass.
In the case of hangers (mechanized breeding), their size determines the number of larvae that can be bred there. From the point of view of the ergonomics of the machinery, it is clear that there can be no mechanized shelves of the size of 15-20 m2 (surface occupied by the larvae in 5th age), but that each frame must be distributed over several shelves. It is also clear that it is not possible, at the time of distribution, to count exactly the number of larvae present per shelf, but that this will be an approximate number, determined by the autonomous movement of the larvae within the surface formed by branches and leaves and intended for their supply.
The number of frames is determined by knowing the surface area approximately occupied by the larvae in each larval age. Furthermore, for a more accurate control in order to determine the number of larvae in the breeding, it is possible to examine samples of pieces, in several points of the breeding, counting the number of larvae that persist in a certain surface. This sampling takes place easily in 5th age. Measure three closed squares of 33 cm x 33 cm = the surface is equal to 0.1 m2. About 100-120 larvae will have to be found inside this square. Sampling must be done at least in three places per piece to be meaningful.
The climb to the wood is also carried out autonomously by the larvae who ambush themselves by climbing on the rays. The number of sunburst is calculated per square meter and the division of the larvae by sunburst is also approximate.
After the breeding, the piece is dragged out of the tunnel / breeding room with itrattori.
Also in this case, it is easy to drag the entire mass of branches with the tractor that make up a long row, while it would be much more complicated and economically advantageous if the mass were divided into separate heaps.
From these technical considerations, it follows that the evaluation of the number of raised telainers must be deduced from the number of incubated frames, from the space occupied
from the breeding itself and from the density of the larvae in the breeding, while a spatial separation of the frames during the breeding is not technically operable, especially if these are numerous.

CRA-API - Apiculture and Bachiculture Research Unit

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